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Flow Cytometer NFC-100 is equipped with dual laser systems emitting light at wavelengths of 488nm and 638nm. It features 6 fluorescence channels allowing for detailed characterization of cells. Our cytometer provides versatility in analyzing a wide range of cell types with particle detection ranging from 0.2 μm to 60 μm. It evaluates at 45,000/sec and processes 96-well plates within 30 minutes, ensuring high-throughput analysis.
| Wavelength | 488nm, 638nm |
| Laser | Semiconductor laser |
| Laser Power |
488 nm & 638 nm laser: 20mW 40/60mW (Optional) |
| Fluorescence Channels | 6 |
| Filters Configuration |
488nm laser: 530/30 nm BP FITC, 585/42 nm BP PE, 700 BP Per CP/ PE-Cy 5, 785/55 nm BP PE-Cy7 638nm laser: 660/20 nm BP APC, 785/55 nm BP APC-Cy7 |
| Optics | The excitation and receiving of solid spatial separation. No fiber conduction. Fixed optical path. |
| Sensitivity of Fluorescence |
FITC < 75 MESF PE < 55 MESF APC < 55 MESF |
| FSC Sensitivity | 0.5 μm |
| SSC Sensitivity | 0.2 μm |
| Range of Particles | 0.2 μm to 60 μm |
| Linear | ≥ 0.98 |
| Resolution | CV ≤2% |
| Carry-Over | ≤ 0.1% |
| Detector | Avalanche Photodiode Detector(APD) |
| Speed | 45,000 events/s; 96 - well plates/30min |
| Minimum Sample Volume | 10μL |
| Speed of Sample | 15 to 235 μL/Min |
| Absolute Count | Volumetric method: Microspheres method |
| Preheating Time | <4min |
| Blending Mode | Whole plate; single pipe; interval mixing is set freely |
| Tube Numbers of Loader | 40 tubes and 96 holes |
| Type of Tube |
Standard flow pipe（12 × 75mm, 5ml) 96 - well plates(F,U,V type) Centrifugal tube (1.5ml, 2ml) |
| Capacity of Liquid Bottle | Waste liquid barrel 6L,Sheath liquid drum 6L,Cleaning liquid drum 1L |
| Maintenance System | Liquid path maintenance automation |
| Software |
Software has an edition in English and Chinese With the function of automatic quality control detection, the software can draw the Levey-Jennings curve automatically Support to be connected with clinical LIS software, to facilitate data graphic transmission |
| Feature of Software |
Automatic saving mode against power failure Detection and analysis can run at the same time |
| Compensation |
Compensation Matrix List Quick Compensation Auto Compensation Auto Compensation Calculation |
| FCS Format | FCS 3.0 |
| Operational Environment | 10°C to 35°C, 15 to 80 % RH |
| Computer Operating System | Win10 pro |
| Voltage | 220 V, 50Hz |
| Dimension (W × D × H ) |
The host system: 44 × 43.5 × 52 cm The host system with leader: 57.2 × 53.3 ×52 cm Liquid tray: 44 × 32 × 45 cm |
| Liquid Tray Weight | 7 Kg |
| Host System Weight | 32 Kg |
| Host System with Leader Weight | 38 Kg |
Flow Cytometer is widely used in immunophenotyping, cell cycle analysis, drug discovery, and hematology.
Flow Cytometer×1
The Apc Detection Board
