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Isocratic HPLC System NHLC-200

Isocratic HPLC System NHLC-200

Isocratic HPLC System NHLC-200 offers a precise flow-rate range of 0.001 to 10 mL/min with ≤±1% accuracy. It operates up to 42.0 MPa, ensuring reliable high-pressure performance. The system features a dual-piston reciprocating pump for stable and consistent solvent delivery. Advanced dual-grating photodiode detection enables accurate full-wavelength scanning for dependable analytical results.


$19,750.00

Catalog

Specifications

Flow Rate Range 0.001 to 10 mL/min
Flow Rate Accuracy ≤ ±1%
Flow Rate Precision RSD < 0.25%
Flow Rate Setting Step 0.001 mL/min
Pump Type Dual-piston reciprocating (in series)
Maximum Operating Pressure 42.0 MPa (adjustable with alarm)
Pressure Pulsation < 0.1 MPa
Display Pressure Error < ±0.2 MPa
Pump Sealing Performance 42 MPa for 10 min, pressure drop < 0.5 MPa
Gradient Capability 2-component or 3-component
Gradient Mode Linear & Step gradient
Gradient Control Internal software / PC & pump control
Wavelength Range 190 to 700 nm
Wavelength Accuracy ±1 nm
Wavelength Repeatability 0.2 nm
Wavelength Step 1 nm
Minimum Detection Limit 2 × 10⁻⁹ g/mL (naphthalene in methanol)
Linear Range 2.8 AU (5%)
Absorbance Range 0.0005 to 4 AU/mV
Baseline Noise ≤ ±0.5 × 10⁻⁵ AU (empty cell)
Baseline Noise (Methanol) ≤ 2 × 10⁻⁵ AU (1 mL/min, 254 nm, 20°C)
Baseline Drift ≤ 0.4 × 10⁻⁴ AU/hr (empty cell)
Baseline Drift (Methanol) ≤ 2 × 10⁻⁴ AU/hr (1 mL/min, 254 nm, 20°C)
Light Source Deuterium lamp / Tungsten lamp
Optical Design Monochromator with dual silicon photodiode
Sample Cell 10 mm optical path, 10 μL volume
Data Output 4 AU/V injection signal, auto zero, dual output
Power Supply AC 110V/220V, 50Hz/60Hz
Detector Power Supply AC 110V/220V, 50Hz/60Hz
Dimensions 400 × 300 × 180 mm
Detector Dimensions 400 x 300 × 180 mm
Packing Dimensions 610 x 420 x 390 mm
Gross Weight 20kg

Features

  • Static motor control with automatic feedback
  • Self-correcting seal and pump head self-cleaning
  • Online plunger seal cleaning
  • Easy flow and pressure calibration
  • Linear and Step gradient modes
  • Programmable solvent compensation
  • Online solvent pre-cleaning
  • High-precision photodiode detection
  • Automatic light intensity calibration
  • Holmium glass for full-spectrum scanning

Applications

  • Isocratic HPLC System NHLC-200 is used for quantitative and qualitative analysis in pharmaceutical, biotechnology, and clinical research laboratories. Suitable for environmental testing, food safety analysis, and chemical quality control applications.

Standard Accessories

UV Detector x 1 Unit

High-Pressure Liquid Pump (Analytical Type) x 1 Unit

Liquid Chromatography Microsyringe x 1 Piece

7725i Manual Injection Valve x 1 Set

C18 Column (4.6 × 250 mm, 5 μm) x 1 Piece

Injection Valve Holder x1 Piece

Startup Tool Kit (Pump System) x 1 Set

Solvent Tray x1 Set

Organic Filter Membrane x1 Box

Syringe Filter Head 0.45 μm (Organic) x 50 Pieces

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