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Flow Cytometer NFC-100
Flow Cytometer NFC-100
Flow Cytometer NFC-100 is a rapid quantitative analytical instrument for enhanced analysis of multiple cell parameters and high throughput sorting of individual cell from a cell population with a high speed linear flow. It is equipped with an avalanche photodiode detector (APD) that converts light-cell interactions into electrical output signal which is then displayed on the monitor in statistical form.
Specifications
| Optical Alignment | Fixed, no operator alignment required |
| Flow pool dimensions | 420×170µm |
| Flow cell material | Quartz |
| Flow cell efficiency | 1.2 NA |
| Scatter Resolution | 0.2 µm |
| Cell size | 0.5-60 µm |
| Detectable granularity range | 0.2-60 µm |
| Fluorescence Resolution | ≤2% CV（fluorescent beads），≤3% CV for CEN |
| Wavelengths | Double laser with 488nm and 638 nm |
| Standard Filter Configuration | Laser (488 nm/638 nm) 6 color analysis |
| Laser | 530 nm BP FITC, 585 nm BP PE,700nmBP Per CP -Cy5.5/PE-Cy5,785nmBP PE-Cy7, 638 lasers: 660 nm BP APC, 785 nm BP APC-Cy7 |
| Heat engine time | <4 minutes |
| Sample Acquisition Rate | 45,000 events/second |
| Sample Flow Rate | 15~ 235μL/Min |
| Sheath Flow Rate | 500mL/h |
| Sample Aspiration Volume | 20µL-5mL |
| Forward sensitivity | 0.5 µm |
| Lateral sensitivity | 0.2 µm |
| Fluid Container Capacity | 6 L sheath, 6 L waste, 250 ml cleaning |
| Carryover | ≤0.1% |
| Dynamic Range | >6 logs ,18 bit; 5.4 decades logarithmic scale |
| Workstation | 23” monitor |
| Computer Operating System | Windows 10 |
| Software | CytoSYS 1.0 |
| Sample loading capacity | 2 × 75 mm tube, 1.5 mL eppendorf tubes |
| Power Requirements | 98-264VAC，50Hz±1Hz |
| Rated Power (W) | 100 W |
| Environmental conditions | Temperature:15-32°C; Relative Humidity: 80% |
| Overall dimensions (W×D×H) | 440×440×520 mm |
| Net Weight | 32Kg |
Features
- ✔ Compact design with stable performance
- ✔ It possess strong specificity, high sensitivity and linearity
- ✔ It allows Multi factor analysis of samples
- ✔ It has function of cell/particle counting
- ✔ It is featured with high performance filter with minima signal loss for transmission and reflection
- ✔ Automated system with initial starting, cleaning and shutdown process
- ✔ Visual compensation tools available for pre/post/live acquisition
- ✔ Optical path with space separation excitation, space separation reception, and no fiber conduction
Applications
It is widely used for analysis of Peripheral blood, bone marrow, fine needle aspirations, solid tissue or adherent cultured cells, microorganisms, microspheres, chromosomes, serum, plasma, supernatant, cell lysate etc for performing various assays like sperm DNA Fragment (DFI), assay of white cells in semen,(CD45), cell apoptosis(Annexi V/PI), acrosome assay(PNA-FIC/PI), urinary and reproductive infection(CD64, cell multifactor, Activated oxygen species for sperms(ROS), round cell contamination, mitochondrial membrane protein, Caspase activation, Th1, Th2 etc.
