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Labnics Automated NGS Library Preparation System NNGS-100


Automated NGS Library Preparation System NNGS-100

What is Automated NGS Library Preparation System NNGS-100?

NNGS-100 is an automated laboratory instrument that prepares DNA and RNA samples for next-generation sequencing (NGS). It performs library preparation steps such as fragmentation, adapter ligation, amplification, and purification with minimal manual intervention, ensuring high consistency and reduced contamination risk.


Quick Answer 

Automated NGS Library Preparation System NNGS-100 is a laboratory automation system that converts DNA or RNA samples into sequencing-ready libraries by automating fragmentation, adapter ligation, amplification, and purification steps.


Key Takeaways

  • Automates NGS library preparation workflow

  • Processes DNA and RNA samples

  • Handles 16–96 samples per run

  • Reduces manual errors and contamination

  • Compatible with standard NGS platforms

  • Used in pharma, biotech, and clinical labs


What Does NNGS-100 Do?

The system prepares sequencing-ready libraries by performing:

  • DNA/RNA fragmentation

  • End repair and adapter ligation

  • PCR amplification

  • Magnetic bead purification

All steps are performed in a controlled automated workflow to ensure reproducibility.


How Does NNGS-100 Work? (Step-by-Step)

Step 1: Sample Loading

DNA or RNA samples are loaded into the system.

Step 2: Fragmentation

Nucleic acids are enzymatically or mechanically fragmented.

Step 3: End Repair & Adapter Ligation

Ends are repaired and sequencing adapters are attached.

Step 4: Amplification

Libraries are amplified via PCR cycles.

Step 5: Purification

Magnetic beads are used to purify final libraries.

Step 6: Output

Sequencing-ready libraries are collected for downstream NGS.


Core Features

  • Automated liquid handling system

  • Programmable multi-step workflows

  • Integrated thermal cycling module

  • Magnetic bead-based purification

  • Sample tracking and barcoding support

  • High reproducibility across batches


Technical Specifications

Parameter

Specification

Sample Input

10 pg – 1 µg

Throughput

16–96 samples/run

Reaction Volume

10–100 µL

Workflow Time

2–4 hours

Purification

Magnetic bead-based

Control System

Digital interface

Power Supply

220V, 50Hz

Weight

~18 kg


Applications (Where It Is Used)

  • Pharmaceutical R&D laboratories

  • Biotechnology research centers

  • Clinical diagnostics labs

  • Genomics sequencing facilities

  • Pathogen detection laboratories

  • Academic research institutions


Why Automation in NGS Library Preparation Matters

Automation improves:

  • Reproducibility across samples

  • Reduction of human error

  • Lower contamination risk

  • Higher throughput efficiency

  • Standardization for clinical compliance


Comparison of Models 

Feature

NNGS-100

NNGS-200

NNGS-300

Throughput

16–96

24–192

48–384

Automation Level

Standard

Advanced

Full workflow

Input Range

10 pg–1 µg

5 pg–2 µg

5 pg–5 µg

Workflow Time

2–4 hrs

3–5 hrs

4–6 hrs

Use Case

Basic automation

Mid-scale labs

High-throughput labs

Which one should you choose?

  • NNGS-100 → Small to mid labs

  • NNGS-200 → Medium research throughput

  • NNGS-300 → Large-scale genomic centers


Industries Using NNGS-100

  • Pharmaceutical companies

  • Biotechnology firms

  • Clinical diagnostic labs

  • Genomics research institutes

  • Epidemiology surveillance labs


Frequently Asked Questions 

1.What is an automated NGS library preparation system?

It is a laboratory instrument that prepares DNA/RNA samples for sequencing by automating library construction steps.

2.What samples can NNGS-100 process?

It processes DNA, RNA, and cDNA samples depending on workflow setup.

3.How many samples can it process at once?

It can process 16 to 96 samples per run.

4.Does it support low-input samples?

Yes, it supports inputs as low as picogram-level DNA/RNA.

5.Is it compatible with sequencing platforms?

Yes, it produces libraries compatible with standard NGS platforms.

6.How long does the process take?

Typically 2–4 hours depending on protocol.

7.Is it suitable for clinical use?

Yes, it supports CLIA, CAP, and GMP-aligned workflows.


Compliance & Standards

  • CLIA guidelines

  • CAP standards

  • GMP-compliant workflows


Alternative Equipment

  • Manual library preparation kits

  • Automated liquid handling systems

  • NGS sequencing platforms

  • QC and library quantification tools


Summary 

Automated NGS Library Preparation System NNGS-100 is designed to streamline DNA and RNA sequencing workflows by fully automating library preparation steps. It enhances reproducibility, reduces manual intervention, and supports scalable genomic research in clinical, pharmaceutical, and biotechnology environments.

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