Digital PCR System NDTP-100 features a single fluorescence channel compatible with both probe and dye detection methods. It supports a standard reaction volume of 15µl for flexible experimental setups. This unit uses a micro-cavity chip with solid-phase segmentation for accurate partitioning. It delivers rapid results with single-chip reading completed in 5 seconds. Our PCR system employs a high-efficiency LED light source for reliable fluorescence detection.
| Number of fluorescence channels | 1 |
|---|---|
| Sample detection throughput | 1/batch, can be read continuously without interruption |
| Daily detection throughput | 360 in a single day (8 hours) |
| Reaction volume | 15µl standard, adjustable within the 40µl range |
| Droplet preparation | Pipetting without an additional microdroplet generation system |
| Sample preparation time | ≤10 seconds/piece |
| Sample detection time | ≤1 minute/piece |
| Detection sensitivity | ≤0.001%, can detect single-copy genes |
| Dynamic range | ≥5 orders of magnitude, 1 to 250000 copies/sample |
| Reagent versatility | Compatible with the probe method and dye method |
| Compatible dyes | FAM, SYBR Green, EvaGreen, VIC/HEX, and other dyes and similar wavelength dyes |
| Supporting reagents | 10× high-concentration DNA detection reagent and 5× RT-dPCR one-step RNA detection reagent |
| Maximum sample input | ≥12 µl |
| Excitation light source | High-efficiency maintenance-free LED light source |
| Detector | High-resolution CMOS sensor |
| Valid time of chip reading | Read repeatedly within 2 weeks |
| Micro-reaction unit | Micro-cavity chip, solid phase segmentation |
| Bio-chip type | 10k, 22k, 120k option, other types are customizable |
| Software | Calculation of copy number, copy number concentration, mutation abundance, confidence interval range, accuracy; threshold line automatic or manual division, single or unified threshold division; output excel data, two-dimensional scatter plot, two-dimensional bar chart, three-dimensional space map; automatically identify complex droplet clusters, output chip actual hole position discrimination map; data quality control function, etc. automatically generate test reports |
| Data security | Permission management, auditing, and electronic signature functions to ensure the validity and reliability of data and meet FDA 21 CFR Part 11 compliance requirements |
| Dimension | Thermal cycler: 480×330×340mm Biochip reader: 130×150×240mm |
| Net weight | Thermal cycler: 8.9kg Biochip reader: 1.7 kg |
Gene amplifier
Biochip reader (1 color)
dPCR biochip box (32 reactions)
Seal oil (100 reactions)
10× Eva dye mix (including UDG) (100 reactions)
10× probe method mix (including UDG) (100 reactions)
One-step mix (100 reactions)
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