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Real Time Thermal Cycler NRTC-100 is a laboratory apparatus that enables to perform reliable molecular biology operations. It is based on Peltier thermal cycle technology that provides efficient protection against physical stresses caused due to deviations in different temperature cycles. It is equipped with Fresnel lens that reduces the light collection of the non target area and thereby enhancing the sensitivity of analysis.
|Sample capacity||0.1ml PCR tubes × 96, 8×12 PCR plate or 96 well plate ×1|
|Reaction volume||10-50 µl|
|Maximum Heating/Cooling rate||6 °C/s|
|Heating Temperature Range||4 °C - 100 °C|
|Temperature Uniformity||±0.2°C at 60°C, ±0.2°C at 95°C|
|Temperature gradients setting range||30°C-100°C|
|Temperature gradient difference setting range||1°C - 36°C|
|Excitation light source||4/6 monochrome high efficiency LEDs|
|Detector||Photomultiplier tube (PMT)|
|Excitation/detection wavelength range||455-650 nm/510-715 nm|
|No. of Fluorescent channels||4|
|Supported dye||FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/ Texas Red, Mustang Purple, Cy5/LIZ|
|Sensitivity||Single copy gene|
|Resolution||1.33 folds copy number difference can be distinguished in single-plex qPCR|
|Dynamic range||10 orders of magnitude copies|
|Overall dimensions||830×750×650 mm|
|Net Weight||22 kg|
It has wide range of applications in different fields as one of the important molecular biology tool. It has multidisciplinary applications in clinical diagnosis, genotyping, gene therapy, regenerative medicines, forensic applications in paternity testing, food safety testing, protein expression studies etc.