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Analytical High-performance Liquid Chromatography NHLC-400

Analytical High-performance Liquid Chromatography NHLC-400 is an advanced, highly effective analytical HPLC system that includes a UV-Vis detector with a wavelength range of 190 to 700 nm, a DAD (Diode Array Detector) detector with a wavelength range of 190 to 800 nm, and a high-pressure constant flow pump and low-pressure quaternary constant flow pump with a flow range of 0.001 to 10.000 mL/min (step 0.001 mL/min). This HPLC system provides accurate, reliable results with improved working efficiency. Powerful software is integrated to regulate, capture, and analyze data, as well as manage the operation

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Specifications

High-Pressure Constant Flow Pump

Flow Range	0.001 to 10.000 mL/min (step 0.001 mL/min)
Accuracy	≤±0.2%
Precision	≤0.06% RSD
Pressure Range	0 to 45 MPa
Power	AC 220 V±10%, 50 Hz/80 W
Dimension (L × W × H)	420 × 300 × 175 mm
Weight	12 kg

Low-Pressure Quaternary Constant Flow Pump

Flow Range	0.001 to 10.000 mL/min (step 0.001 mL/min)
Gradient Accuracy	≤±1%
Retention Time Repeatability	≤0.1%
Maximum Working Pressure	63 MPa
Communication Methods	UDP Network Communication
Mixed Volume	750μL
Power	AC 220 V±10%, 50 Hz/100 W
Dimension (L × W × H)	400 × 300 × 180 mm
Weight	15 kg

UV-Vis Detector

Linear	2.0 AU
Wavelength Range	190 to 700 nm
Noise	≤± 0.5,105 AU
Drift	≤1.0 × 104 AU/h
Cell	Analytical: Optical path 10 mm, 11μL Semi-prep scale: Optical path 2 mm, 6μL
Power	AC 220V±10%, 50 Hz/100 W
Dimension (L × W × H)	420 × 300 × 175 mm
Weight	16 kg

Diode Array Detector

Linear	≥2.0 AU
Wavelength Range	190 to 800 nm
Noise	≤±1.0M05 AU
Drift	≤2.0 × 104 AU/h
Power	AC220 V±10%, 50 Hz/180 W
Dimension (L × W × H)	500 × 300 × 175 mm
Weight	18 kg

Column oven

Temperature Range	Ambient +5°C to 85 °C
Accuracy	±0.3 °C
Precision	≤0.1 °C
Power	AC220 V±10%, 50 Hz/130 W
Dimension (L × W × H)	420 × 300 × 120 mm
Weight	7kg

Other parameters

Pump	P3100 × 2
Detector	UV3100 × 1
Gradient mixer	TD-1-15 × 1
Solvent bottle tray	TP3100 × 1
Sample injector	Rheodyne 7725i × 1
Column	Supersil ODS2 5pm ID4.6 × 200mm × 1
Workstation	W5100 × 1
Valve mounting frame	ZJ-1 × 1
System kit	EClassical 3100 × 1
Power	AC220V±10%, 50Hz
Package size	600 × 440 × 380 mm × 3
Gross weight	45 kg

Features

✔ Comprehensive and Flexible System Configuration
✔ Pumping system: high-pressure constant flow pump, low-pressure quaternary constant flow pump
✔ Equipped with the isocratic system, binary gradient system, and quaternary gradient system
✔ Advanced USB chip and optoelectronic isolation ensure stable communication
✔ Powerful software for controlling, capturing, and analyzing data, as well as managing operations
✔ Real-time pressure display, along with maximum and minimum pressure warnings, ensures safety

Applications

It is widely used to separate and analyze a mixture of compounds in multidisciplinary fields like research and development studies, food science, environmental science for analysis and detection of impurities, ligand-exchange chromatography, high-pH anion-exchange chromatography of carbohydrates and oligosaccharides, water purification, pre-concentration of trace components, and ion-exchange chromatography of proteins